| 1 | 1 | Dave Martinsky, Dave Summerton, Haley Brogan, Alicia Kueker | U40 | 1:01:37 | Analyze |
| 2 | 2 | Kara Mobly, Maria Fecik, Christopher Balven, Juan Herrera | U40 | 1:01:54 | Analyze |
| 3 | 3 | Keaton Nelligan, Jessica Sarni, Taylor Roberts, Kaleb Yates | U40 | 1:04:00 | Analyze |
| 4 | 4 | Britton Ezell, Conner Netherland, Mallory Meredith, Allison Motto | U40 | 1:04:34 | Analyze |
| 5 | – | Yaakoby Jordan, Hayden Voss, Fabian Acuna, Ruth Sandescu | – | 1:09:11 | Analyze |
| 6 | 5 | Jenna Littmann, Kayla Cittadino, Kiefer Calisto, Hajime Ichikawa | U40 | 1:10:21 | Analyze |
| 7 | 6 | Mario Bermudez, Clara Nolasco, Katherine Bermudez, Marvin Rodriguez | U40 | 1:12:14 | Analyze |
| 8 | 1 | Sara Ahmad, Tom Wilson, Ryan Moody, Megan May | 40+ | 1:12:38 | Analyze |
| 9 | 7 | Nicki Forte, Robert Lee, Daniel Ochoa, Stephanie Arreola-Perez | U40 | 1:13:24 | Analyze |
| 10 | 8 | Amanda Alexander, Noah Islas, Gabriel Garza, Cassie Mcclure | U40 | 1:14:42 | Analyze |
| 11 | 9 | Mykal Hogue, Lex Poole, Brianna Warren, Chinweuba Nkenke | U40 | 1:15:38 | Analyze |
| 12 | 10 | Caroline O'Driscoll, Kole Boucher, Jeremy Rodriguez, Mary Worth | U40 | 1:16:21 | Analyze |
| 13 | 11 | Blaine Blackburn, Brooke Smith, Mitch Scarmardo, Lauren Babin | U40 | 1:17:15 | Analyze |
| 14 | 12 | Valentina Michelena, Judge Hansen, Peyton Ford, Paige Crawford | U40 | 1:18:50 | Analyze |
| 15 | 13 | Laura Derks, Vernell Robinson, Greg Guse, Debrenda Nash | U40 | 1:18:58 | Analyze |
| 16 | 14 | Marc Seah, Eric Hu, Mi Rae Lee, Emily Hu | U40 | 1:20:13 | Analyze |
| 17 | 15 | Emme Rhodes, Jackson Malcolm, Saxon Stroud, Morgan Powell | U40 | 1:20:52 | Analyze |
| 18 | 16 | George Blevins, Ashlee James, Yeicob Calderon, Amy Harenberg | U40 | 1:24:06 | Analyze |
| 19 | 2 | Michael Warber, Ana Alvarez, Vanessa Murrell, Jake Munta | 40+ | 1:24:53 | Analyze |
| 20 | 17 | Robert Goodin, Jacob Batchelor, Kristina Boyle, Courtney Snukis | U40 | 1:25:05 | Analyze |
| 21 | 3 | Jason Adams, Edward Irizzary, Delta Gutierrez-Garcia, Gabriela Navarrete | 40+ | 1:26:10 | Analyze |
| 22 | 4 | Ray Morris, Monica Jangrus, Logan Hayter, Erin Morris | 40+ | 1:26:42 | Analyze |
| 23 | 18 | Angela Guffey, Bryce Guffey, Rachel Barden, Kyle Barden | U40 | 1:28:22 | Analyze |
| 24 | 19 | Tulia Taborga-Urquiola, Steven Gunawan, Josh Carrasco, Andrea Carrasco | U40 | 1:29:22 | Analyze |
| 25 | 20 | Josh Lawson, Raegen Martin, Jenny Lawson, Chase Garsee | U40 | 1:29:41 | Analyze |
| 26 | 21 | Lynn Notarianni, Rolando Celis, Miguel Millan, Jadira Jazmine Varela | U40 | 1:29:48 | Analyze |
| 27 | 22 | Jordan Babcock, Maggie Babcock, Will Valdez, Madison Valdez | U40 | 1:30:02 | Analyze |
| 28 | 23 | Hershell Harvey, Ella Horne, Linton Brown, Tavia Garvey | U40 | 1:30:35 | Analyze |
| 29 | 24 | Cathy Hensley, Bridget Hillseth, Hernaniz Herrera, Don Rebsamen | U40 | 1:31:02 | Analyze |
| 30 | 25 | Sarah Mumma, Natalie Thomas, Rob Montealvo, Kweku Arkorful | U40 | 1:32:37 | Analyze |
| 31 | 5 | Heather Liner, John Wallet, John Krueger, Lauren Miller | 40+ | 1:32:48 | Analyze |
| 32 | 26 | Kimberly Senni, Sophia Neang, Mark Schoenemann, Peter Neang | U40 | 1:32:58 | Analyze |
| 33 | 27 | Rachel Wood, Ashley Golemon, Tyler Smith, STEVEN SIMPSON | U40 | 1:33:00 | Analyze |
| 34 | 28 | Meghan Malone, Grant Bergeron, Heather Bush, Cameron Dean | U40 | 1:33:34 | Analyze |
| 35 | 29 | Christine Rand, Charissa Stewart, Ricky Chatman, Jonathan Arrington | U40 | 1:33:38 | Analyze |
| 36 | 30 | Nubia Millan, Molly Walton, Jesus Cadena, Kelsey Thompson | U40 | 1:34:12 | Analyze |
| 37 | 31 | Grant Weller, Victoria Poplin, Madison Carter, Garrett Hite | U40 | 1:34:19 | Analyze |
| 38 | 32 | Teresa Ontiveros, Julio Ontiveros, Julio César Ontiveros Jr, Veronica Ontiveros | U40 | 1:36:48 | Analyze |
| 39 | 33 | Diana Vila, Daniel Cabungcal, Heather Horsley, Will Mayes | U40 | 1:38:20 | Analyze |
| 40 | 34 | Douglas Smith, Sydney Darbe, Cristina Patton, Benjamin Darbe | U40 | 1:38:36 | Analyze |
| 41 | 6 | Philip Mantaring, Peter Mantaring, Monika Veleva, Sue Covert | 40+ | 1:38:46 | Analyze |
| 42 | 35 | Marcellis Wade, Victor Umeh, Xochitl Alvarez, Cynthia Ileso | U40 | 1:38:49 | Analyze |
| 43 | 36 | Jose Moreno, Anna Stevens, Lauren Reed, Chuck Goff | U40 | 1:39:18 | Analyze |
| 44 | 7 | Valerie Wilson, Jose Vega, Vanessa Gonzalez, Scott Wilson | 40+ | 1:39:46 | Analyze |
| 45 | 37 | Allison Corona Del Cid, Jake Lundy, Zophia Corona Del Cid, Edward Mitri | U40 | 1:40:28 | Analyze |
| 46 | 38 | Curtis Okafor, Victoria Adesanya, Jasmin Thompson, Devyn Oatis | U40 | 1:43:36 | Analyze |
| 47 | 39 | Nicolas Arredondo, Albert Valverde, Sharon Joseph, Janell Gambrell | U40 | 1:48:13 | Analyze |
| 48 | 40 | Vanessa Nzeh, Glad Nwaozo, Trenton Orr, Kingsley Nwaozo | U40 | 1:48:18 | Analyze |
| 49 | 41 | Keesha Partida, Jason Suber, Mark De Leon, Brook Varner | U40 | 1:54:13 | Analyze |
| 50 | 8 | Justin Fordyce, Jennifee Fordyce, Jim Fordyce, Kooper Briley | 40+ | 1:55:56 | Analyze |
| 51 | 9 | Douglas Williams, Sheila Willis, Greta Cambridge, Jason Leslie | 40+ | 2:01:48 | Analyze |
| 52 | 42 | Shandrea Middlebrook, Rikia Ancar, Brandon Blake, Quintarus Thomas | U40 | 2:12:37 | Analyze |
| 53 | 43 | Joshua Adesanya, Nana Thompson, Mary Walker, Vivian Adesanya | U40 | 2:26:36 | Analyze |