| 1 | 1 | Lauren Rantala, Marc Howe | 30-39 | 58:44 | Analyze |
| 2 | 2 | Christine Chapman, Stephen Levall | 30-39 | 1:06:38 | Analyze |
| 3 | 1 | Leah Casey, Finn Merrill | 16-29 | 1:07:02 | Analyze |
| 4 | 3 | Leo Armenta, Brittany Howard | 30-39 | 1:07:57 | Analyze |
| 5 | 4 | Bill Brumbach, Joy Brumbach | 30-39 | 1:08:28 | Analyze |
| 6 | 5 | Brandon Bonine, Brittany Bonine | 30-39 | 1:09:07 | Analyze |
| 7 | 6 | Erin Meyers, Jon Coluzzi | 30-39 | 1:09:11 | Analyze |
| 8 | 1 | Stephanie Danielson, Christopher Madden | 40-49 | 1:09:49 | Analyze |
| 9 | 2 | Dina Duy, Jeremy Tangerose | 40-49 | 1:10:37 | Analyze |
| 10 | 7 | Charlie Cullen, Anna Cunningham | 30-39 | 1:11:45 | Analyze |
| 11 | 2 | Taylor Lavella, Andrew Glatt | 16-29 | 1:11:52 | Analyze |
| 12 | 8 | Stephanie Hunter, Russell Mock | 30-39 | 1:11:55 | Analyze |
| 13 | 3 | Rocco Rasnic, Angela Zurales | 16-29 | 1:12:08 | Analyze |
| 14 | 1 | Carie Kretschmer, John Kretschmer | 50-59 | 1:13:37 | Analyze |
| 15 | 4 | Hannah Drake, Bradley Cowan | 16-29 | 1:14:10 | Analyze |
| 16 | 9 | Brad Pullins, Caitlyn Pullins | 30-39 | 1:15:29 | Analyze |
| 17 | 10 | Trevor Thomas, Sarah Gonsiorowski | 30-39 | 1:16:37 | Analyze |
| 18 | 11 | Igor Gryniv, Megan Vinicky | 30-39 | 1:16:45 | Analyze |
| 19 | 12 | Samir Khezzar, Alexandria Frazer | 30-39 | 1:16:52 | Analyze |
| 20 | 3 | Dani Dunham, Ricky Rosado | 40-49 | 1:18:21 | Analyze |
| 21 | 4 | Maureen Gray, Ken Gray | 40-49 | 1:18:43 | Analyze |
| 22 | 5 | Hailey Bryant, Jalen Bryant | 16-29 | 1:19:57 | Analyze |
| 23 | 6 | Arturo Ayala, Mariana Pino | 16-29 | 1:20:07 | Analyze |
| 24 | 13 | Cody Mcmillen, Valerie Mcmillen | 30-39 | 1:22:17 | Analyze |
| 25 | 7 | Caleb Labelle, Emily Gray | 16-29 | 1:22:33 | Analyze |
| 26 | 14 | Ryan Hart, Rory Scher | 30-39 | 1:22:35 | Analyze |
| 27 | – | Bryce Wood, Amira Rajab | – | 1:22:52 | Analyze |
| 28 | 15 | Gavin Bruce, Gabrielle Bruce | 30-39 | 1:23:06 | Analyze |
| 29 | 16 | Luke Mol, Ashley Cullen-Mol | 30-39 | 1:24:29 | Analyze |
| 30 | 8 | Kaitlyn Cooper, Justin Bogedin | 16-29 | 1:24:37 | Analyze |
| 31 | 17 | Joshua Marlow, Yaritza Nieves | 30-39 | 1:24:37 | Analyze |
| 32 | 2 | Scott Smilie, Kristy Moran | 50-59 | 1:26:20 | Analyze |
| 33 | 5 | Natalie Bedell, Brent Bedell | 40-49 | 1:26:42 | Analyze |
| 34 | 18 | Danny Griffin, Sari Griffin | 30-39 | 1:27:51 | Analyze |
| 35 | 6 | Will Dammann, Stephanie Seymour | 40-49 | 1:27:59 | Analyze |
| 36 | 19 | Angela Orlowski, Bryan Fratianne | 30-39 | 1:28:32 | Analyze |
| 37 | 20 | Cavalas Blanks, Rebecca Blanks | 30-39 | 1:28:50 | Analyze |
| 38 | 21 | Alissa Long, Nathan Long | 30-39 | 1:32:12 | Analyze |
| 39 | 22 | Travis Powell-Wolf, Katie Pechous | 30-39 | 1:32:58 | Analyze |
| 40 | 23 | Kaila Osborne, Kevin Kottke | 30-39 | 1:33:41 | Analyze |
| 41 | 9 | Harkirat Jawanda, Miranda Viars | 16-29 | 1:38:02 | Analyze |
| 42 | 24 | Lindsey Boden, Ryan Wolff | 30-39 | 1:40:12 | Analyze |
| 43 | 7 | Johanna De Castro, Jason Moulton | 40-49 | 1:40:47 | Analyze |
| 44 | 25 | Thomas Wilgenbusch, Alexa Wilgenbusch | 30-39 | 1:41:10 | Analyze |
| 45 | 1 | Helen Torchia, Christopher Torchia | 60-69 | 1:41:19 | Analyze |
| 46 | 8 | Amy Latawiec, Joe Hakim | 40-49 | 2:20:26 | Analyze |
| 47 | – | Johnny Range, Emine Baliyska | – | 2:23:27 | Analyze |