| 1 | 1 | Sara Pascale, Christopher Balven, Kara Mobly, Zane Landry | 40+ | 1:09:35 | Analyze |
| 2 | 2 | Noah Huisman, Angela Gargano, Cynthia Thorne, Kendall Gunier | 40+ | 1:09:44 | Analyze |
| 3 | 3 | Tanya Torres, Daniel Lyons, Jordan English, Connor Penn | 40+ | 1:11:03 | Analyze |
| 4 | 4 | Hannah White, Zach Morgan, Hayleigh White, Carson Omilusik | 40+ | 1:11:18 | Analyze |
| 5 | 1 | Danielle Russell, Christopher Everett, Jennifer Gallery, Josh Widener | 40+ | 1:12:02 | Analyze |
| 6 | 5 | Tye Orr, Natalee Watson, Rob Garfio, Sarah Garritson | 40+ | 1:12:51 | Analyze |
| 7 | 2 | Julie Pittman, Katy Hardaway, Ubaldo Lopez, Jorge Gama | 40+ | 1:15:10 | Analyze |
| 8 | 6 | Jason Sergent, Marisa Davila, Bernadette Gutierrez, Vanilo Beaudouin | 40+ | 1:16:56 | Analyze |
| 9 | 3 | Matt Childs, Joe Ostiguy, Sara Sprawls, Sonja Griffin | 40+ | 1:18:59 | Analyze |
| 10 | 7 | Steve Stitle, Anthony Robinette, Whitney Broyles, Jarynn Stewart | 40+ | 1:20:05 | Analyze |
| 11 | 4 | Gary Scott, Chad Hensley, Tasha Smith, Brenda Edwards | 40+ | 1:22:13 | Analyze |
| 12 | 8 | Joe Lowery, Sasha Jones, Ryan Calhoun, Lizet Rendon | 40+ | 1:22:35 | Analyze |
| 13 | 9 | Lakendrick King, Bruce Taylor, Teresa Assama, Taylor Farmon | 40+ | 1:22:48 | Analyze |
| 14 | 5 | Mary Jackson, Kris Zschiesche, Natalie Robertson, Tommy Cole | 40+ | 1:23:00 | Analyze |
| 15 | 10 | Nick Garcia, Jonathan Johnson, Shannon Wright, Courtney Goodman | 40+ | 1:24:56 | Analyze |
| 16 | 6 | Heather Gatewood, Jaeme Simmons, Brian Simmons, Joe Green | 40+ | 1:26:03 | Analyze |
| 17 | 11 | Michael Trozzo, Ashley Shugart, Jordan Bates, Kristin Gonzalez | 40+ | 1:26:12 | Analyze |
| 18 | 12 | Michael Kerzee, Andrew Howell, Callie Howell, Emily Lemons | 40+ | 1:26:35 | Analyze |
| 19 | 13 | Brian Anderson, Rachel Levin, Javier Santos, Jade Jameson | 40+ | 1:30:10 | Analyze |
| 20 | 14 | Amir Khan, Monica Jimenez, Tyler Wade, Yadira Hernandez | 40+ | 1:31:53 | Analyze |
| 21 | 15 | Guy Wilson, Brian Elwell, Jessica Elwell, Theresia Mcintire | 40+ | 1:33:52 | Analyze |
| 22 | 16 | Brandon Casarez, Dan Ragan, Sandra Hernandez, Cindy Chesumbai | 40+ | 1:36:06 | Analyze |
| 23 | 17 | Terrelle Smith, Stephanie Williams, Riana Turner, Jamal Phillips | 40+ | 1:39:55 | Analyze |
| 24 | 18 | Elvira Resendez, Josh Daly, Marissa Jones, Brandon Woodstock | 40+ | 1:42:01 | Analyze |
| 25 | 7 | George Cleveland, Mike Serdar, Jennifer Carling, Norma Williams | 40+ | 1:53:09 | Analyze |
| 26 | 19 | Winfred Gbedema, Nana Thompson, Ezinne Nwankwo, Victoria Adesanya | 40+ | 1:56:03 | Analyze |
| 27 | 8 | David Chapman, Loretta Francis, Rachel Lam, Steve Lam | 40+ | 2:00:27 | Analyze |